1차 2차

타이틀

ORIGINAL ARTICLE : THREE-DIMENSIONAL BIOPRINTING OF THICK VASCULARIZED TISSUES

게시판 상세보기
작성일 2016-04-15 조회수 25
16-04-15 10:10

The advancement of tissue and, ultimately, organ engineering requires the ability to pattern human
tissues composed of cells, extracellular matrix, and vasculature with controlled microenvironments
that can be sustained over prolonged time periods.

To date, bioprinting methods have yielded thin tissues that only survive for short durations.
To improve their physiological relevance, we report a method for bioprinting 3D cell-laden, vascularized
tissues that exceed 1 cm in thickness and can be perfused on chip for long time periods (>6 wk).

Specifically, we integrate parenchyma, stroma, and endothelium into a single thick tissue by coprinting
multiple inks composed of human mesenchymal stem cells (hMSCs) and human neonatal dermal
fibroblasts (hNDFs) within a customized extracellular matrix alongside embedded vasculature, which is
subsequently lined with human umbilical vein endothelial cells (HUVECs).

These thick vascularized tissues are actively perfused with growth factors to differentiate hMSCs toward
an osteogenic lineage in situ. This longitudinal study of emergent biological phenomena in complex
microenvironments represents a foundational step in human tissue generation.
 

첨부파일
이전 다음 글보기
이전글 [ORIGINAL ARTICLE] A 3D BIOPRINTING SYSTEM TO PRODUCE HUMAN-SCALE TISSUE CONSTRUCTS WITH STRUCTURAL INTEGRITY
다음글 DRUG MADE WITH 3-D PRINTER WINS FIRST FDA APPROVAL
맨위로