The advancement of tissue and, ultimately, organ engineering requires the ability to pattern human
tissues composed of cells, extracellular matrix, and vasculature with controlled microenvironments
that can be sustained over prolonged time periods.
To date, bioprinting methods have yielded thin tissues that only survive for short durations.
To improve their physiological relevance, we report a method for bioprinting 3D cell-laden, vascularized
tissues that exceed 1 cm in thickness and can be perfused on chip for long time periods (>6 wk).
Specifically, we integrate parenchyma, stroma, and endothelium into a single thick tissue by coprinting
multiple inks composed of human mesenchymal stem cells (hMSCs) and human neonatal dermal
fibroblasts (hNDFs) within a customized extracellular matrix alongside embedded vasculature, which is
subsequently lined with human umbilical vein endothelial cells (HUVECs).
These thick vascularized tissues are actively perfused with growth factors to differentiate hMSCs toward
an osteogenic lineage in situ. This longitudinal study of emergent biological phenomena in complex
microenvironments represents a foundational step in human tissue generation.