To determine the cell viability during the printing process, survival of 3T3 fibroblasts in the 3-D bioprinted structures was examined. Live/dead cell assays showed ≥ 90% cell survival on day 0, which was maintained through days 4 and 7. Data indicate that the Gel4Cell® maintained the cell viability during the printing process and provided a proper microenvironment for cell proliferation.
- Cell source: 3T3 cells (1x106 cells/ml)
- Scan speed : 120 mm/min
- Nozzle size: 300 µm Teflon nozzle
The bioprinted constructs made by Gel4Cell® maintained their structure with time. To evaluate structural stability of the Gel4Cell® in culture condition, dimension of the printed constructs were analyzed. Approx. 70% of dimension change was observed at 21 days after printing.